ISOLATION OF NUCLEIC ACIDS

The minced (cut into small pieces) tissue is treated with 1M NaCl solution to extract nucleoproteins. The extract containing nucleoproteins is saturated with NaCl when proteins and nucleic acids are separated from each other; the proteins are actually precipitated out during this operation. This are removed and the nucleic acids are precipitated from the filtrate with alcohol.
The mixture of RNA and DNA is extracted with phenol with dissolves only RNA, leaving behind DNA. Differential centrifugation, ion exchange chromatography and other technique can also be used for separating DNA and RNA.

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